Cloning of some heat shock proteins genes for further transcriptional study of Planktothrix agardhii exposed to abiotic stress
Planktothrix agardhii is one of the freshwater cyanobacteria that can
produce the hepatotoxin microcystins (MC)—a real threat to human and
animal health. Knowledge of the biological role of MC in producing
organisms is highly desired to understand the driving force of MC
production. Recently, emerging evidences have suggested that MC may have
protective role in cells facing environmental stress. If this is true,
one should expect differences in the cellular protective mechanisms
between MC-containing and MC-deficient mutant strains. To test this
hypothesis, it would be essential to investigate the consequences of the
loss of MC in Planktothrix in the transcriptional responses of its heat
shock proteins (Hsps) to abiotic stresses—an important component of
cellular stress response. However, a crucial first step is prerequisite
for the isolation of hsp genes here, as the genome of Planktothrix has
not been fully published. Therefore, we have successfully isolated four
hsp genes including clpC (hsp100), htpG (hsp90), groEL (hsp60), and
groES (hsp10) from Planktothrix agardhii PCC 7805 using ramped annealing
PCR (RAN-PCR) with consensus-degenerate hybrid oligonucleotide primers
(CODEHOP) and annealing control primer (ACP) system. In addition, some
putative regulatory sequences found in the upstream region of groESL
operon of Planktothrix agardhii were also discussed.
| Title: | Cloning of some heat shock proteins genes for further transcriptional study of Planktothrix agardhii exposed to abiotic stress |
| Authors: | Tran, Chi Thi Du Bernard, Cécile Comte, Katia |
| Keywords: | Animalia;heat shock protein;bacterial DNA;cyanobacterium;Molecular |
| Issue Date: | 2015 |
| Publisher: | Kluwer Academic Publishers |
| Citation: | Scopus |
| Abstract: | Planktothrix agardhii is one of the freshwater cyanobacteria that can produce the hepatotoxin microcystins (MC)—a real threat to human and animal health. Knowledge of the biological role of MC in producing organisms is highly desired to understand the driving force of MC production. Recently, emerging evidences have suggested that MC may have protective role in cells facing environmental stress. If this is true, one should expect differences in the cellular protective mechanisms between MC-containing and MC-deficient mutant strains. To test this hypothesis, it would be essential to investigate the consequences of the loss of MC in Planktothrix in the transcriptional responses of its heat shock proteins (Hsps) to abiotic stresses—an important component of cellular stress response. However, a crucial first step is prerequisite for the isolation of hsp genes here, as the genome of Planktothrix has not been fully published. Therefore, we have successfully isolated four hsp genes including clpC (hsp100), htpG (hsp90), groEL (hsp60), and groES (hsp10) from Planktothrix agardhii PCC 7805 using ramped annealing PCR (RAN-PCR) with consensus-degenerate hybrid oligonucleotide primers (CODEHOP) and annealing control primer (ACP) system. In addition, some putative regulatory sequences found in the upstream region of groESL operon of Planktothrix agardhii were also discussed. |
| Description: | Folia Microbiologica, Volume 60, Issue 4, 25 December 2015, Pages 317-323 |
| URI: | http://repository.vnu.edu.vn/handle/VNU_123/29876 |
| ISSN: | 00155632 |
| Appears in Collections: | Bài báo của ĐHQGHN trong Scopus |
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