Chemical modification of silicon nitride microsieves for capture of MCF-7 circulating tumor cells of breast cancer

Chemical modification of silicon nitride (SiN) microsieves with glutaraldehyde and 3-glycidoxypropyldimethylethoxysilane (GOPS) for bio-coupling with an antibody to capture MCF-7 circulating tumor cells of breast cancer is reported. In this research, the antibody monoclonal anti-cytokerantin-FITC with fluorescein isothiocyanate label was used due to its good selectivity to MCF-7 circulating tumor cells of breast cancer. Modification efficiency was determined by the variation of contact angle. The increase in contact angle of the microsieves treated with glutaraldehyde and GOPS indicated that the microsieve surface changed from hydrophilic to hydrophobic. These results confirmed the successful immobilization of glutaraldehyde and GOPS onto SiN microsieves. Antibody binding effect was evaluated by fluorescence microscopy. Fluorescent images exhibited that GOPS was more effective than the glutaraldehyde treatment. The GOPS-treated microsieves were then used for capture of MCF-7 cells in phosphate buffered saline (PBS). The fluorescent images proved that the surface modification of SiN microsieves with GOPS helped to increase the efficiency of MCF-7 capture.

Title:	Chemical modification of silicon nitride microsieves for capture of MCF-7 circulating tumor cells of breast cancer
Authors:	To, Thien Dien Le, Huyen Thoai Dinh, Mai Ngoc Thi Nguyen, Anh Tuan Doan, Tin Chanh Duc Dang, Chien Mau
Keywords:	Breast cancer Classification numbers: 2.04;Glutaraldehyde;GOPS;MCF-7;Silicon nitride;Surface modification
Issue Date:	2015
Publisher:	Institute of Physics Publishing
Citation:	Scopus
Abstract:	Chemical modification of silicon nitride (SiN) microsieves with glutaraldehyde and 3-glycidoxypropyldimethylethoxysilane (GOPS) for bio-coupling with an antibody to capture MCF-7 circulating tumor cells of breast cancer is reported. In this research, the antibody monoclonal anti-cytokerantin-FITC with fluorescein isothiocyanate label was used due to its good selectivity to MCF-7 circulating tumor cells of breast cancer. Modification efficiency was determined by the variation of contact angle. The increase in contact angle of the microsieves treated with glutaraldehyde and GOPS indicated that the microsieve surface changed from hydrophilic to hydrophobic. These results confirmed the successful immobilization of glutaraldehyde and GOPS onto SiN microsieves. Antibody binding effect was evaluated by fluorescence microscopy. Fluorescent images exhibited that GOPS was more effective than the glutaraldehyde treatment. The GOPS-treated microsieves were then used for capture of MCF-7 cells in phosphate buffered saline (PBS). The fluorescent images proved that the surface modification of SiN microsieves with GOPS helped to increase the efficiency of MCF-7 capture.
Description:	Advances in Natural Sciences: Nanoscience and Nanotechnology, Volume 6, Issue 2, 1 June 2015, Article number 025006
URI:	http://iopscience.iop.org/article/10.1088/2043-6262/6/2/025006/meta http://repository.vnu.edu.vn/handle/VNU_123/32326
ISSN:	20436262
Appears in Collections:	Bài báo của ĐHQGHN trong Scopus